Components(final concentrations)
2M Tris base
1 M acetate
100mM EDTA
H2O
To make 1 liter(usually kept as stock and diluted when used)
242g Tris base
57.1 mL glacial acetic acid (17.4M) N.B: GLACIAL ACETIC ACID WILL BURN YOU! USE IN HOOD AND AVOID ALL CONTACT! If in doubt about how to handle glacial acetic acid speak to your mentor first!
100 mL of .5M EDTA(pH 8.0)
H2O up to a liter
(1X TAE pH 8.1 is 40mM Tris, 20mM acetate and 2mM EDTA)
use at 1X
EDTA: P/N: OMNI-PUR 4050
TRIS BASE: P/N: SIGMA T1503-500G
GLACIAL ACETIC ACID: P/N: SIGMA-ALDRICH 695084-2.5L